PEOPLE > Andy Hoofnagle, M.D., Ph.D.

Andy Hoofnagle, M.D., Ph.D.

Professor of Laboratory Medicine & Pathology;
KRI Laboratory Director

Dept. of Laboratory Medicine
University of Washington
Box 357110
1959 NE Pacific St.
Room NW120
Seattle, WA 98195-7110

P: 206-598-4005

F: 206-598-6189


Research Interests

There are three main directions of research in our laboratory. The first investigates the interaction of inflammation and lipid metabolism using proteomics, mouse models, and cell-based methods. In addition, we are developing novel high-throughput immunoassays and quantitative mass spectrometric assays for biomarker validation in large studies. The second is the development of clinical laboratory tests using liquid-chromatography/tandem mass spectrometry. Initially we are attempting to clinically translate peptide immunoaffinity methodologies to the detection in serum of the tumor marker thyroglobulin, the best marker we have for differentiated thyroid carcinoma. Lastly, we use informatics and data mining to develop useful benchmarks from large datasets of common laboratory tests to evaluate medical interventions throughout the hospital to optimize patient care and to ensure patient safety.


Hoofnagle AN, Becker JO, Wener MH, and Heinecke JW. (2008) Quantification of thyroglobulin, a low-abundance serum protein, by immunoaffinity peptide enrichment and tandem mass spectrometry. Clin Chem. 54:1796-804.

Marney LC, Laha TJ, Baird GS, Rainey PM, and Hoofnagle AN. (2008) Isopropanol protein precipitation for the analysis of plasma free metanephrines by liquid chromatography-tandem mass spectrometry. Clin Chem. 54:1729-32.

Hoofnagle AN, Peterson GN, Kelly JL, Sayre CA, Chou D, and Hirsch IB. (2008) Use of serum and plasma glucose measurements as a benchmark for improved hospital-wide glycemic control. Endocr Pract. 14:556-63.

Vaisar T, Pennathur S, Green PS, Gharib SA, Hoofnagle AN, Cheung MC, Byun J, Vuletic S, Kassim S, Singh P, Chea H, Knopp RH, Brunzell J, Geary R, Chait A, Zhao1 X, Elkon K, Marcovina S, Ridker P, Oram JF, and Heinecke JW. (2007) Shotgun Proteomics Implicates Protease Inhibition and Complement Activation in the Anti-inflammatory Properties of HDL. J Clin Invest. 117:746-56.

Hoofnagle AN, Chou D, and Astion ML. (2007) Online Database for Documenting Clinical Pathology Resident Education. Clin Chem. 53:134-7.

Hoofnagle AN, Laha TJ, Rainey PM, and Sadrzadeh SMH. (2006) Specific detection of anabasine, nicotine and nicotine metabolites in urine by liquid chromatography-tandem mass spectrometry. Am J Clin Path. 126:880-7.

Pierce KM, Hoggard JC, Hope JL, Rainey PM, Hoofnagle AN, Jack RM, Wright BW, and Synovec RE. (2006) Fisher Ratio Method Applied to Third-Order Separation Data To Identify Significant Chemical Components of Metabolite Extracts. Anal Chem. 78:5068-75.

Hoofnagle AN and Wener MH. (2006) Serum thyroglobulin: A model of immunoassay imperfection. Clin Lab Int. 12:12-4.

Hoofnagle AN, Stoner JW, Lee T, Eaton SS, and Ahn, NG. (2004) Phosphorylation-dependent changes in structure and dynamics in ERK2 detected by SDSL and EPR. Biophys J. 86:395-403.

Hoofnagle AN, Resing KA, and Ahn NG. (2003) Protein Analysis by Hydrogen Exchange Mass Spectrometry. Annu Rev Biophys Biomol Struct. 32:1-25.

Emrick MA, Hoofnagle AN, Miller AS, Ten Eyck LF, and Ahn NG. (2001) Constitutive activation of extracellular signal-regulated kinase 2 by synergistic point mutations. J Biol Chem. 276:46469-79.

Hoofnagle AN, Resing KA, Goldsmith EJ, and Ahn NG. (2001) Changes in protein conformational mobility upon activation of extracellular regulated protein kinase-2 as detected by hydrogen exchange. Proc Natl Acad Sci U S A. 98:956-61.